Millbrook was the tobacco estate of John Wayles Eppes in Buckingham County, Virginia. In 1810, six years after the death of his wife, Maria Jefferson Eppes, John Wayles Eppes sold Eppington plantation, in Chesterfield County, and moved to Millbrook.
Eppes's move to Buckingham County was politically strategic. Thomas Jefferson wanted his son-in-law to run for Congress to oust a political nemesis, John Randolph. Eppes succeeded in doing this in the 1813 election. It was at Millbrook that Eppes married his second wife, Martha Burke Jones, in 1809.
The estate had a two-story house, which burned in 1866.